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Developmental ability of cloned embryos from neural stem cells

¹ Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan, ² Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan, ³ Department of Life Science, Graduate School of Science and Technology, Kobe University, Kobe 657–8501, Japan and ⁴ Research Unit for Cell Plasticity, Center for Developmental Biology, RIKEN, Kobe, Japan

Correspondence should be addressed to E Mizutani; Email: mizutani{at}cdb.riken.jp

The success rate is generally higher when cloning mice from embryonic stem (ES) cell nuclei than from somatic cell nuclei, suggesting that the embryonic nature or the undifferentiated state of the donor cell increases cloning efficiency. We assessed the developmental ability of cloned embryos derived from cultured neural stem cell (NSC) nuclei and compared the success rate with that of embryos cloned from other donor cells such as differentiated NSCs, cumulus cells, Sertoli cells and ES cells in the mouse. The transfer of two-cell cloned embryos derived from cultured NSC nuclei into surrogate mothers produced five live cloned mice. However, the success rate (0.5%) was higher in embryos cloned from cultured NSC nuclei than from differentiated NSCs (0%), but lower than that obtained by cloning mice from other cell nuclei (2.2–3.5%). Although the in vitro developmental potential to the two-cell stage of the cloned embryos derived from NSC nuclei (73%) was similar to that of the cloned embryos derived from other somatic cell nuclei (e.g., 85% in Sertoli cells and 75% in cumulus cells), the developmental rate to the morula–blastocyst stage was only 7%. This rate is remarkably lower than that produced from other somatic cells (e.g., 50% in Sertoli cells and 54% in cumulus cells). These results indicate that the undifferentiated state of neural cells does not enhance the cloning efficiency in mice and that the arrest point for in vitro development of cloned embryos depends on the donor cell type.

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