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SSLP-1, a secreted Ly-6 protein purified from mouse seminal vesicle fluid

¹ Department of Medical Research, Mackay Memorial Hospital, Tamshui 251, Taiwan, ROC, ² Mackay Medicine, Nursing and Management College, Taipei 112, Taiwan, ROC, ³ Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei 104, Taiwan, ROC, ⁴ Digitalgene Biosciences Co., Ltd., Taipei 114, Taiwan, ROC, and ⁵ Genomics Research Center, Academia Sinica, Taipei 115, Taiwan, ROC

Correspondence should be addressed to S-H Li; Email: lsh{at}ms1.mmh.org.tw

The Ly-6 protein family refers to a group of glycophosphatidyl inositol-anchored membrane proteins with ten conserved cysteines. They are thought to be involved in cellular adhesion and signaling. Recently, a subfamily of secreted Ly-6 proteins has been identified. In the present study, we report a secreted Ly-6 protein, secreted seminal vesicle Ly-6 protein 1 (SSLP-1) purified from mouse seminal vesicles using a series of steps including ion-exchange chromatography on a diethylaminoethyl (DEAE)-Sephacel column, gel filtration on a Sephadex G-75 column, and ion-exchange HPLC on a sulfopropyl column. Further analysis demonstrated it to be a novel, previously unnamed, 17 kDa glycoprotein. N-glycosidase F treatment revealed a core protein with a molecular mass of 8720 Da. By Basic Local Alignment Search Tool Protein analysis, we found that SSLP-1 had ten conserved cysteine residues identical with other secreted Ly-6 proteins. The gene Gm191, which is located on chromosome 9, encodes SSLP-1. By Northern blotting with 21 different mouse tissues, we found that Sslp-1 mRNA was predominantly expressed in the seminal vesicle. Immunohistochemistry revealed SSLP-1 protein in the luminal fluid and mucosal epithelium of the seminal vesicles. The amount of Sslp-1 mRNA and SSLP-1 protein in the seminal vesicle was regulated by testosterone and correlated with the stage of animal maturation. The tissue-specific expression pattern suggests that SSLP-1 may play a physiological role in male mouse reproduction.

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