This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhao, M.-r. Articles by Wang, Y.-l. Search for Related Content PubMed PubMed Citation Articles by Zhao, M.-r. Articles by Wang, Y.-l.

Dual effect of transforming growth factor ß1 on cell adhesion and invasion in human placenta trophoblast cells

¹ State Key Laboratory of Reproductive Biology, ² State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Road, Beijing 100080, People’s Republic of China and ³ Graduate School of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China

Correspondence should be addressed to Y-L Wang; Email: wangyl{at}ioz.ac.cn

Transforming growth factor ß (TGFß) has been shown to be a multifunctional cytokine required for embryonic development and regulation of trophoblast cell behaviors. In the present study, a non-transformed cell-line representative of normal human trophoblast (NPC) was used to examine the effect of TGFß1 on trophoblast cell adhesion and invasion. In vitro assay showed that TGFß1 could significantly promote intercellular adhesion, while inhibiting cell invasion across the collagen I-coated filter. Reverse transcription (RT)-PCR and gelatin zymography demonstrated that TGFß1 evidently repressed the mRNA expression and proenzyme production of matrix metalloproteinase (MMP)-9, but exerted no effect on mRNA expression and secretion of MMP-2. On the other hand, both the mRNA and protein expression of epithelial-cadherin and ß-catenin were obviously upregulated by TGFß1 in dose-dependent fashion, as revealed by RT-PCR and western-blot analysis. What is more, one of the critical TGFß signaling molecules – Smad2 was notably phosphorylated in TGFß1-treated NPC cells. The data indicates that cell invasion and adhesion are coordinated processes in human trophoblasts and that there exists paracrine regulation on adhesion molecules and invasion-associated enzymes in human placenta.

This article has been cited by other articles:

				S. M. Nelson and I. A. Greer The potential role of heparin in assisted conception Hum. Reprod. Update, November 1, 2008; 14(6): 623 - 645. [Abstract] [Full Text] [PDF]