Reproduction  
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS  
Reproduction (2006) 131 1115-1126
DOI: 10.1530/rep.1.01062
Copyright © 2006 Society for Reproduction and Fertility
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vonnahme, K. A
Right arrow Articles by Grazul-Bilska, A. T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vonnahme, K. A
Right arrow Articles by Grazul-Bilska, A. T

RESEARCH

Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2{alpha}-induced regression in sheep

Kimberly A Vonnahme1,2,3, Dale A Redmer1,2,3, Ewa Borowczyk1, Jerzy J Bilski1,2, Justin S Luther1, Mary Lynn Johnson1,2,3, Lawrence P Reynolds1,2,3 and Anna T Grazul-Bilska1,2,3

1 Department of Animal and Range Sciences, 2 Cell Biology Center and 3 Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA

Correspondence should be addressed to A T Grazul-Bilska; Email: Anna.Grazul-Bilska{at}ndsu.edu

Corpora lutea and blood samples were collected from superovulated ewes 0, 4, 8, 12 and 24 h after prostaglandin F2{alpha} (PGF) analog injection on day 10 of the estrous cycle. Changes in vascular cell and fibroblast composition, apoptosis and mRNA expression for several angiogenic factors in the corpus luteum (CL) were determined. While peripheral progesterone concentration decreased at 24 h after PGF injection, CL weight did not change. The area of positive BS-1 lectin staining (endothelial cell marker), smooth muscle cell actin (SMCA; pericyte and SMC marker), collagen type 1 (fibroblast marker), and the rate of cell death changed in luteal tissues after PGF treatment. In association with these cellular changes, mRNA for several angiogenic factors including vascular endothelial growth factor (VEGF) and receptors (Flt and KDR), basic fibroblast growth factor (FGF2) and receptor, angiopoietin (ANGPT) 1 and receptor Tie-2, endothelial nitric oxide synthase (NOS3), and angiotensin II receptor 1 (AT1) were altered. Changes in endothelial cell marker expression were positively correlated with changes in VEGF and NO systems. In addition, changes in mRNA expression for VEGF, Flt and KDR were positively correlated with changes in ANGPT2, Tie-2, and NOS3, indicating a functional relationship. This data demonstrates that after an initial increase, the endothelial component of the vascular bed decreases during PGF-induced luteal regression. However, SMCA expression remained high during luteal regression, potentially indicating a role of pericytes and vascular SMC in luteolysis, likely to regulate tissue remodeling and to maintain the integrity of larger blood vessels. Further, it appears that early regression may increase collagen type 1 production and/or expression by fibroblasts. Expression of angiogenic factors is influenced by PGF-induced luteolysis and may serve to maintain vascular structure in order to aid luteal regression.




This article has been cited by other articles:


Home page
 ReproductionHome page
A. T Grazul-Bilska, J. S Caton, W. Arndt, K. Burchill, C. Thorson, E. Borowczyk, J. J Bilski, D. A Redmer, L. P Reynolds, and K. A Vonnahme
Cellular proliferation and vascularization in ovine fetal ovaries: effects of undernutrition and selenium in maternal diet
Reproduction, April 1, 2009; 137(4): 699 - 707.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K.A. Vonnahme, W.J. Arndt, M.L. Johnson, P.P. Borowicz, and L.P. Reynolds
Effect of Morphology on Placentome Size, Vascularity, and Vasoreactivity in Late Pregnant Sheep
Biol Reprod, November 1, 2008; 79(5): 976 - 982.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
M. R. O'Neil, G. P. Lardy, L. P. Reynolds, J. S. Caton, and K. A. Vonnahme
Impacts of linseed meal and estradiol-17{beta} on mass, cellularity, angiogenic factors, and vascularity of the jejunum
J Anim Sci, November 1, 2008; 86(11): 3014 - 3022.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
J.-M. Li, M. Mogi, J. Iwanami, L.-J. Min, K. Tsukuda, A. Sakata, T. Fujita, M. Iwai, and M. Horiuchi
Temporary Pretreatment With the Angiotensin II Type 1 Receptor Blocker, Valsartan, Prevents Ischemic Brain Damage Through an Increase in Capillary Density
Stroke, July 1, 2008; 39(7): 2029 - 2036.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. Shirasuna, S. Watanabe, T. Asahi, M. P B Wijayagunawardane, K. Sasahara, C. Jiang, M. Matsui, M. Sasaki, T. Shimizu, J. S Davis, et al.
Prostaglandin F2{alpha} increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum: the possible regulation of blood flow at an early stage of luteolysis
Reproduction, April 1, 2008; 135(4): 527 - 539.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
A. T. Grazul-Bilska, C. Navanukraw, M. L. Johnson, K. A. Vonnahme, S. P. Ford, L. P. Reynolds, and D. A. Redmer
Vascularity and expression of angiogenic factors in bovine dominant follicles of the first follicular wave
J Anim Sci, August 1, 2007; 85(8): 1914 - 1922.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A. T Grazul-Bilska, C. Navanukraw, M. L. Johnson, D. A Arnold, L. P Reynolds, and D. A Redmer
Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle.
Reproduction, October 1, 2006; 132(4): 579 - 587.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS  
Copyright © 2006 by the Society for Reproduction and Fertility.