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Reproduction (2006) 131 929-942
DOI: 10.1530/rep.1.00657
Copyright © 2006 Society for Reproduction and Fertility
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RESEARCH

Modulation of peroxisome proliferator-activated receptor {delta} and {gamma} transcripts in swine endometrial tissue during early gestation

Etienne Lord, Bruce D Murphy, Joëlle A Desmarais, Sandra Ledoux, Danièle Beaudry1 and Marie-France Palin1

Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 7C6 and 1 Dairy and Swine Research and Development Center, Agriculture and Agri-Food Canada, PO Box 90, 2000 Route 108 East, Lennoxville, Quebec, Canada J1M 1Z3

Correspondence should be addressed to M-F Palin; Email: palinmf{at}agr.gc.ca

Recent evidence points to a role for peroxisome proliferator-activated receptors (PPARs) {delta} and {gamma} in embryo implantation and survival. In this study, we report the porcine PPAR{delta} complete coding sequence and mRNA abundance of PPAR{delta}, PPAR{gamma}1 and {gamma}2, angiopoietin-like protein 4 (ANGPTL4) and adipocyte determination and differentiation-dependent factor 1 (ADD1) genes in the pregnant sow endometrium. Real-time PCR analysis was used to study the effect of parity (Yorkshire-Landrace multiparous (YL) and nulliparous (YLn)), site of endometrial tissue sampling (between and at embryo attachment sites) in crossbred DurocxYorkshire-Landrace (DYL) sows and stages of pregnancy (non-pregnant, day 15 and day 25 after mating) in Meishan-Landrace (ML) on mRNA levels. Parity effects were observed for PPAR{delta}, ANGPTL4, and ADD1, with higher mRNA levels in YL than YLn sows. In DYL sows, lower mRNA levels were present at attachment sites compared to between attachment sites for PPAR{delta}, PPAR{gamma}1, and ANGPTL4. Finally, day 15 pregnant ML sows had lower PPAR{delta} mRNA levels compared to day 15 cycling ML sows. A significant increase of PPAR{gamma}1 mRNA levels was found on day 25 pregnant ML and DYL sows relative to day 15 ML or DYL pregnant sows. PPAR{delta} and {gamma} immunostaining was detected in endometrial tissue of day 15 cycling sows, day 15 and 25 pregnant sows and epithelial cells of day 25 embryos. Collectively, our results suggest a role for PPAR{delta}, PPAR{gamma}1, and ANGPTL4, but not PPAR{gamma}2, during the peri-implantation period in pregnant sows.




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