This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cannon, M. J Articles by Pate, J. L Search for Related Content PubMed PubMed Citation Articles by Cannon, M. J Articles by Pate, J. L

Presence and regulation of messenger ribonucleic acids encoding components of the class II major histocompatibility complex-associated antigen processing pathway in the bovine corpus luteum

Department of Animal Sciences, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691, USA and ¹ Olson Center for Women’s Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA and Veterans Affairs Medical Center, Omaha, Nebraska, 68105, USA

Correspondence should be addressed to J L Pate; Email: pate.1{at}osu.edu

Luteal cells express class II major histocompatibility complex (MHC) molecules and can stimulate T lymphocyte proliferation in vitro. However, it is unknown whether luteal cells express the intracellular components necessary to process the peptides presented by class II MHC molecules. The objective of the present study was to examine the expression and regulation of three major class II-associated antigen processing components – class II MHC-associated invariant chain (Ii), DM and DMß – in luteal tissue. Corpora lutea were collected early in the estrous cycle, during midcycle and late in the estrous cycle, and at various times following administration of a luteolytic dose of prostaglandin F₂ (PGF₂) to the cow. Northern analysis revealed the presence of mRNA encoding each of the class II MHC-associated antigen processing proteins in luteal tissue. Ii mRNA concentrations did not change during the estrous cycle, whereas DM and DMß mRNA concentrations were highest in midcycle luteal tissue compared with either early or late luteal tissue. Tumor necrosis factor- (TNF-) reduced DM mRNA concentrations in cultured luteal cells in the presence of LH or PGF₂. DM and DMß mRNA were also present in highly enriched cultures of luteal endothelial (CLENDO) cells, and DM mRNA concentrations were greater in CLENDO cultures compared with mixed luteal cell cultures. Expression of invariant chain, DM and DMß genes indicates that cells within the corpus luteum express the minimal requirements to act as functional antigen-presenting cells, and the observation that CLENDO cells are a source of DM and DMß mRNA indicates that non-immune cells within the corpus luteum may function as antigen-presenting cells.

This article has been cited by other articles:

				M. J Cannon, J. S Davis, and J. L Pate The class II major histocompatibility complex molecule BoLA-DR is expressed by endothelial cells of the bovine corpus luteum Reproduction, May 1, 2007; 133(5): 991 - 1003. [Abstract] [Full Text] [PDF]

				D. H. Townson Immune cell-endothelial cell interactions in the bovine corpus luteum Integr. Comp. Biol., December 1, 2006; 46(6): 1055 - 1059. [Abstract] [Full Text] [PDF]