This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Berisha, B. Articles by Schams, D. Search for Related Content PubMed PubMed Citation Articles by Berisha, B. Articles by Schams, D.

Changes in fibroblast growth factor 2 and its receptors in bovine follicles before and after GnRH application and after ovulation

Physiology, Technical University of Munich, Weihenstephaner Berg 3, D-85350 Freising, Germany and ¹ Anatomy and Physiology, University of Hohenheim, Stuttgart, Germany

Correspondence should be addressed to D Schams; Email: physio{at}wzw.tum.de

The aim of this study was to evaluate the expression pattern of fibroblast growth factor 2 (FGF2), its receptor variants (FGFR1IIIc, FGFR2IIIc) and nucleolin in time-defined follicle classes before and after GnRH application and after ovulation in the cow. Ovaries containing preovulatory follicles or new corpora lutea (CL) were collected at approximately 0, 4, 10, 20 and 25 h (follicles) and 60 h (new CL) relative to injection of GnRH to induce an LH surge (n = 5 animals per group). The expressions of FGF2 and FGFR1IIIc mRNA were significantly up-regulated only in the follicle group 4 h after GnRH (during the LH surge) with a significant down-regulation immediately afterwards. Western blot analyses showed two protein bands with at 22 and 18 kDa with apparent up-regulation beginning with the LH surge (4 h) and maximum levels 20 h after GnRH. FGF2 protein in follicles collected at 0 h (before LH surge) was localised in theca tissue (endothelial and pericytes of blood vessels) but not in granulosa cells (GCs). The FGF2 staining (by immunohistochemistry) pattern changed dramatically after the LH surge for a short period (about 2 days) and FGF2 protein was localised dominantly in the nucleus of many GCs, while most capillary endothelial cells were FGF2 immunonegative. In conclusion, the novel observation of FGF2 up-regulation and the distinct change in FGF2 localisation from theca (cytoplasm of endothelial cells) to the nucleus of GCs after the LH surge may be important for survival of GCs or for the transition of the GCs to luteal cells.

This article has been cited by other articles:

				R S Robinson, K J Woad, A J Hammond, M Laird, M G Hunter, and G E Mann Angiogenesis and vascular function in the ovary Reproduction, December 1, 2009; 138(6): 869 - 881. [Abstract] [Full Text] [PDF]

				P. Humaidan, E.G. Papanikolaou, and B.C. Tarlatzis GnRHa to trigger final oocyte maturation: a time to reconsider Hum. Reprod., October 1, 2009; 24(10): 2389 - 2394. [Abstract] [Full Text] [PDF]

				K. J Woad, A. J Hammond, M. Hunter, G. E Mann, M. G Hunter, and R. S Robinson FGF2 is crucial for the development of bovine luteal endothelial networks in vitro Reproduction, September 1, 2009; 138(3): 581 - 588. [Abstract] [Full Text] [PDF]

				D. Schams, V. Steinberg, M. Steffl, H. H D Meyer, and B. Berisha Expression and possible role of fibroblast growth factor family members in porcine antral follicles during final maturation Reproduction, July 1, 2009; 138(1): 141 - 149. [Abstract] [Full Text] [PDF]

				J. A. Grado-Ahuir, P. Y. Aad, G. Ranzenigo, F. Caloni, F. Cremonesi, and L. J. Spicer Microarray analysis of insulin-like growth factor-I-induced changes in messenger ribonucleic acid expression in cultured porcine granulosa cells: Possible role of insulin-like growth factor-I in angiogenesis J Anim Sci, June 1, 2009; 87(6): 1921 - 1933. [Abstract] [Full Text] [PDF]

				E. Klipper, E. Tatz, T. Kisliouk, I. Vlodavsky, U. Moallem, D. Schams, Y. Lavon, D. Wolfenson, and R. Meidan Induction of Heparanase in Bovine Granulosa Cells by Luteinizing Hormone: Possible Role during the Ovulatory Process Endocrinology, January 1, 2009; 150(1): 413 - 421. [Abstract] [Full Text] [PDF]

				R S Robinson, A J Hammond, G E Mann, and M G Hunter A novel physiological culture system that mimics luteal angiogenesis Reproduction, March 1, 2008; 135(3): 405 - 413. [Abstract] [Full Text] [PDF]

				H Kliem, H Welter, W D Kraetzl, M Steffl, H H D Meyer, D Schams, and B Berisha Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum Reproduction, September 1, 2007; 134(3): 535 - 547. [Abstract] [Full Text] [PDF]

				R.S. Robinson, L.T. Nicklin, A.J. Hammond, D. Schams, M.G. Hunter, and G.E. Mann Fibroblast Growth Factor 2 Is More Dynamic than Vascular Endothelial Growth Factor A During the Follicle-Luteal Transition in the Cow Biol Reprod, July 1, 2007; 77(1): 28 - 36. [Abstract] [Full Text] [PDF]