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A rat model for functional characterization of pregnancy-induced denervation and postpartum reinnervation in the myometrium and cervix: a superfusion study

Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary

Correspondence should be addressed to G Falkay, Szeged, Eötvös u. 6, H-6721, Hungary; Email: falkay{at}pharma.szote.u-szeged.hu

The pregnancy-induced rapid degeneration of the adrenergic nerves innervating the uterus is a well-known but poorly understood phenomenon. Since most of the published investigations were carried out by histological assay, our aim was to describe the loss of the adrenergic function during pregnancy and the reinnervational procedure in the postpartum period. Myometrial and cervical samples from rats were loaded with [³H]noradrenaline and then transferred into a chamber for superfusion. After a wash-out period, fractions were collected. The fifth and fifteenth fraction tissues were stimulated with an electric field. The [³H]noradrenaline contents of the fractions were determined, together with the amount remaining in the tissue. The myometrial [³H]noradrenaline release was substantially decreased in early pregnancy, and absent in the late stage. Differences in release profile were detected between the implantation sites and the interimplantation areas. As a refinement of the results of previous histochemical studies, the noradrenergic functions of the cervix were found to be deeply affected in the early postpartum period. The pregnancy-induced denervational procedure can be followed by means of a superfusional technique after [³H]noradrenaline loading. As our technique is considered to be similar in sensitivity to histological methods, superfusion can be regarded as a model for functional investigations of pregnancy-induced denervation.