This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dehghani, H. Articles by Hahnel, A. C Search for Related Content PubMed PubMed Citation Articles by Dehghani, H. Articles by Hahnel, A. C

Expression profile of protein kinase C isozymes in preimplantation mouse development

Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, N1G 2W1 Canada

Correspondence should be addressed to A C Hahnel; Email: ahahnel{at}ovc.uoguelph.ca

In the preimplantation mouse embryo, the protein kinase C (PKC) family has been implicated in regulation of egg activation, progression of meiotic and mitotic cell cycles, embryo compaction, and blastulation, but the involvement of the individual isozymes is largely unknown. Here, using semiquantitative immunocytochemistry and confocal microscopy we analyze the relative amount and subcellular distribution of ten isozymes of PKC (, ßI, ßII, , , , , , , /) and a PKC-anchoring protein, receptor for activated C-kinase 1 (RACK1). Our results show that all of these isoforms of PKC are present between the two-cell and blastocyst stages of mouse preimplantation development, and that each has a distinct, dynamic pattern and level of expression. The data suggest that different complements of the isozymes are involved in various steps of preimplantation development, and will serve as a framework for further functional studies of the individual isozymes. In particular, there was a transient increase in the nuclear concentration of several isozymes at the early four-cell stage, suggesting that some of the PKC isozymes might be involved in regulation of nuclear organization and function in the early mouse embryo.

This article has been cited by other articles:

				H. Dehghani, C. Reith, and A. C Hahnel Subcellular localization of protein kinase C {delta} and {varepsilon} affects transcriptional and post-transcriptional processes in four-cell mouse embryos Reproduction, October 1, 2005; 130(4): 453 - 465. [Abstract] [Full Text] [PDF]