| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
RESEARCH |
1 Center for Animal Biotechnology and Genomics and Department of Animal Science and 2 Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA
Correspondence should be addressed to T E Spencer; Email: tspencer{at}tamu.edu
Galectin-15 is the newest member of a secreted ß-galactoside-binding lectin family. The galectin-15 gene is expressed specifically by the endometrial luminal epithelium (LE) and superficial ductal glandular epithelium (sGE) of the ovine uterus. The proposed extracellular role of secreted galec7tin-15 is to regulate implantation and placentation by functioning as a heterophilic cell adhesion molecule between the conceptus trophectoderm and endometrial LE, while that of intracellular galectin-15 is to regulate cell survival, differentiation and function. The present study determined galectin-15 expression in uteroplacental tissues during gestation and in the postpartum uterus. In the uterine lumen, secreted galectin-15 was found as multimers, particularly on days 14 and 16 of pregnancy. In the endometrial epithelium and conceptus trophectoderm, intracellular galectin-15 protein was found associated with crystalline structures. Between days 20 and 120 of pregnancy, galectin-15 mRNA was expressed specifically by the LE and sGE of the intercaruncular endometrium of ewes. Immunoreactive galectin-15 protein was most abundant in the trophectoderm with lower levels in the endometrial LE and sGE. Galectin-15 protein was detected in allantoic fluid, but not in amniotic fluid. After parturition, galectin-15 mRNA declined in the endometrium from postpartum day (PPD) 1 to 28 and exhibited a variegated expression pattern in the LE and sGE. These results indicate that galectin-15 is synthesized and secreted throughout gestation by the endometrial LE/sGE and is absorbed by the placenta and forms crystals within the trophectoderm, whereas the remainder is cleared into the allantois after being transported into the fetal circulation via the placental areolae. Based on the biological properties of other galectin family members, galectin-15 is hypothesized to have biological roles in conceptus–endometrial interactions, uterine immune and inflammatory responses, and placental morphogenesis and function.
This article has been cited by other articles:
 |
|
 |
|
  J. L. Farmer, R. C. Burghardt, F. D. Jousan, P. J. Hansen, F. W. Bazer, and T. E. Spencer Galectin 15 (LGALS15) functions in trophectoderm migration and attachment FASEB J, February 1, 2008; 22(2): 548 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K Lewis, J. L Farmer, R. C Burghardt, G. R Newton, G. A Johnson, D. L Adelson, F. W Bazer, and T. E Spencer Galectin 15 (LGALS15): A Gene Uniquely Expressed in the Uteri of Sheep and Goats that Functions in Trophoblast Attachment Biol Reprod, December 1, 2007; 77(6): 1027 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G Lea and O. Sandra Immunoendocrine aspects of endometrial function and implantation Reproduction, September 1, 2007; 134(3): 389 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B A Quinn, M A Hayes, R O Waelchli, M W Kennedy, and K J Betteridge Changes in major proteins in the embryonic capsule during immobilization (fixation) of the conceptus in the third week of pregnancy in the mare Reproduction, July 1, 2007; 134(1): 161 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Satterfield, F. W. Bazer, and T. E. Spencer Progesterone Regulation of Preimplantation Conceptus Growth and Galectin 15 (LGALS15) in the Ovine Uterus Biol Reprod, August 1, 2006; 75(2): 289 - 296. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
