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Reproduction (2005) 130 61-69
DOI: 10.1530/rep.1.00549
Copyright © 2005 Society for Reproduction and Fertility
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RESEARCH

Cytoskeleton localization in the sperm head prior to fertilization

Katerina Dvoráková, Harry D M Moore1, Natasa Sebková and Jirí Palecek

Department of Developmental Biology, Faculty of Science, Charles University of Prague, Vinicna 7, 12844 Prague 2, Czech Republic and 1 Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK

Correspondence should be addressed to K Dvorakova; Email: k_dvorak{at}natur.cuni.cz

Three major cytoskeletal proteins, actin, tubulin and spectrin, are present in the head of mammalian spermatozoa. Although cytoskeletal proteins are implicated in the regulation of capacitation and the acrosome reaction (AR), their exact role remains poorly understood. The aim of this study was to compare the distribution of the sperm head cytoskeleton before and after the AR in spermatozoa representing a range of acrosome size and shape. Spermatozoa from the human and three rodents (rat, hamster and grey squirrel) were fixed before and after the AR in appropriate medium in vitro. Indirect immunofluorescent localization of cytoskeletal proteins was undertaken with antibodies recognizing actin, spectrin and {alpha}-tubulin. Preparations were counterstained with propidium iodide and examined by epifluorescent and confocal microscopy. Our results clearly demonstrated changes in localization of cytoskeleton during the AR, mainly in the apical acrosome with further changes to the equatorial segment and post-acrosomal regions. The pattern of cytoskeletal proteins in the sperm head of all the species was similar in respect to various sub-compartments. These observations indicated that the sperm head cortical cytoskeleton exhibits significant changes during the AR and, therefore, support the image of cytoskeletal proteins as highly dynamic structures participating actively in processes prior to fertilization.




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