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Effect of LH on prostaglandin F₂ and prostaglandin E₂ secretion by cultured porcine endometrial cells

Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, Olsztyn 10-747, Poland

Correspondence should be addressed to A Blitek; Email: agas{at}pan.olsztyn.pl

LH appears to be a potent stimulator of the release of endometrial prostaglandins (PGs) in the pig. The aim of the present studies was to examine the effect of LH on PGF₂ and PGE₂ secretion by cultured porcine endometrial cells on days 10–12 and 14–16 of the oestrous cycle and to compare its action with oxytocin. A time-dependent effect of LH (10 ng/ml) on PGF₂ release from luminal epithelial and stromal cells on days 10–12 was observed (experiment 1). The highest increase in PGF₂ secretion in response to LH was detected in stromal cells after 6 h of incubation (P < 0.001). Epithelial cells responded to LH after a longer exposure time (P < 0.01). A concentration-dependent effect of LH (0.1–100 ng/ml) on PGF₂ release from stromal cells was examined after 6 h and from epithelial cells after 12 h (experiment 2). Effective concentrations of LH were 10 and 100 ng/ml. LH (10 ng/ml) and oxytocin (100 nmol/l) affected PGF₂ and PGE₂ secretion from endometrial cells on days 10–12 and 14–16 of the oestrous cycle (experiment 3). LH stimulated PGF₂ secretion from both cell types and its action was more potent on days 10–12. LH induced PGE₂ release, especially in epithelial cells on days 14–16. A stimulatory effect of oxytocin on PGF₂ was confirmed in stromal cells, but this hormone was also shown to enhance PGE₂ output. These results indicated that LH, like oxytocin, a very effective stimulator of PGF₂ release, could play an important role in the induction of luteolysis.

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