Reproduction   citetrack
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS  
Reproduction (2005) 130 1-13
DOI: 10.1530/rep.1.00685
Copyright © 2005 Society for Reproduction and Fertility
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by White, C. A
Right arrow Articles by Salamonsen, L. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by White, C. A
Right arrow Articles by Salamonsen, L. A

REVIEW

A guide to issues in microarray analysis: application to endometrial biology

Christine A White1,2 and Lois A Salamonsen1

1 Prince Henry’s Institute of Medical Research, PO Box 5152, Clayton, Victoria, 3168, Australia and 2 Dept of Obstetrics & Gynaecology, Monash University, Clayton, Victoria, 3168, Australia

Correspondence should be addressed to C A White; Email: christine.white{at}phimr.monash.edu.au

Within the last decade, the development of DNA microarray technology has enabled the simultaneous measurement of thousands of gene transcripts in a biological sample. Conducting a microarray study is a multi-step process; starting with a well-defined biological question, moving through experimental design, target RNA preparation, microarray hybridisation, image acquisition and data analysis – finishing with a biological interpretation requiring further study. Advances continue to be made in microarray quality and methods of statistical analysis, improving the reliability and therefore appeal of microarray analysis for a wide range of biological questions. The purpose of this review is to provide both an introduction to microarray methodology, as well as a practical guide to the use of microarrays for gene expression analysis, using endometrial biology as an example of the applications of this technology. While recommendations are based on previous experience in our laboratory, this review also summarises the methods currently considered to be best practice in the field.




This article has been cited by other articles:


Home page
 ReproductionHome page
S L Rodriguez-Zas, K Schellander, and H A Lewin
Biological interpretations of transcriptomic profiles in mammalian oocytes and embryos
Reproduction, February 1, 2008; 135(2): 129 - 139.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
J.A. Horcajadas, A. Pellicer, and C. Simon
Wide genomic analysis of human endometrial receptivity: new times, new opportunities
Hum. Reprod. Update, January 1, 2007; 13(1): 77 - 86.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
Z. He, W.-Y. Chan, and M. Dym
Microarray technology offers a novel tool for the diagnosis and identification of therapeutic targets for male infertility
Reproduction, July 1, 2006; 132(1): 11 - 19.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS  
Copyright © 2005 by the Society for Reproduction and Fertility.