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Reproduction (2004) 128 819-828
DOI: 10.1530/rep.1.00244
Copyright © 2004 Society for Reproduction and Fertility
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RESEARCH

Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

Sayyed Mohammad Hadi Alavi1, Jacky Cosson2, Mahmoud Karami1, Bagher Mojazi Amiri1 and Mohammd Ali Akhoundzadeh3

1 Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, PO Box 31585-4314, Karaj, Iran 2 Biologie du Développement, UMR 7009 CNRS, Université Pierre et Marie Curie, Observatoire Océanologique, 06 234 Villefranche sur Mer Cedex, France and 3 Shahid Beheshti Artificial Sturgeon Propagation and Rearing Center, PO Box 3117, Rasht, Iran

Correspondence should be addressed to Sayyed Mohammad Hadi Alavi; Email: smhadi_alavi{at}yahoo.com

Sperm motility is a prerequisite factor determining semen quality and fertilizing capacity. The effects of environmental factors including pH, cations and osmolality as well as the role of dilution rate on sperm motility parameters in Acipenser persicus were studied. The best pH and dilution rate for activation of spermatozoa were pH 8.0 and dilution ratio 1:50. Ionic factors can stimulate the initiation of sperm activation. The maximum percentage of motile sperm and total duration of sperm motility were observed in solutions containing 25 mM NaCl, 0.2 mM KCl, 3 mM CaSO4, 10 mM MgSO4 and sucrose with an osmolality of 50 mosmol kg–1. The present study provides us with some basic knowledge about sturgeon spermatozoa biosensitivity to ionic and osmolality effects. A sensitivity of A. persicus sperm was observed after induction of activation of sperm motility in solution containing cations or sucrose with high osmolality. Concentrations more than 50 mM Na+, more than 1 mM K+, more than 3 mM Ca2+ and more than 10 mM Mg2+ had negative effects on sperm motility. Also, osmolality more than 100 mosmol kg–1 had an inhibitory effect. It is clear that ions and osmolality stimulate the motility of spermatozoa by changes in the properties of the plasma membrane including its potential and its ionic conductance. The inhibitory role of high osmolality of the swimming medium (more than 100 mosmol kg–1) and insufficient osmolality of the seminal plasma to inhibit semen motility suggested that osmolality is not the principal factor preventing sperm motility in seminal fluid but that K+ is a major inhibitory factor of sperm motility in seminal plasma.




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L. Zilli, R. Schiavone, C. Storelli, and S. Vilella
Molecular Mechanisms Determining Sperm Motility Initiation in Two Sparids (Sparus aurata and Lithognathus mormyrus)
Biol Reprod, August 1, 2008; 79(2): 356 - 366.
[Abstract] [Full Text] [PDF]


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