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Reproduction (2004) 128 717-725
DOI: 10.1530/rep.1.00335
Copyright © 2004 Society for Reproduction and Fertility
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RESEARCH

Exogenous interferon-{gamma} alters murine inner cell mass and trophoblast development. Effect on the expression of ErbB1, ErbB4 and heparan sulfate proteoglycan (perlecan)

Vanina Fontana, Virginia Choren, Liliana Vauthay1, Juan Carlos Calvo2,3, Lucrecia Calvo and Monica Cameo

Laboratorio Biología de la Reproducción, Ecuador 1465 2°B (1425), 1 Instituto de Oncología A.H. Roffo (UBA), 2 Instituto de Biología y Medicina Experimental and 3 Department of Biological Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina

Correspondence should be addressed to M Cameo; Email: monicameo{at}fibertel.com.ar

Implantation is a crucial event in human pregnancy. The participation of cytokines in the implantation process has been widely documented, although the role of many of these molecules is still a matter of controversy. In a previous report from our laboratory, we demonstrated that addition of interferon-{gamma} to the culture medium produces deleterious effects on mouse embryo development. In this study we investigated the effect of this cytokine on outgrowing embryo morphology and on the expression of epidermal growth factor receptors (ErbBs) and heparan sulfate proteoglycan (perlecan) in mouse embryos cultured in vitro. Morphological assessment of inner cell mass and trophoblast development was carried on in-situ fixed and stained outgrowths. Localization of ErbB1, ErbB4 and perlecan on pre- and peri-implantation embryos was investigated by immunocytochemistry. Addition of interferon-{gamma} produced a deleterious effect on both inner cell mass and trophoblast morphology. Immunostaining demonstrated that ErbB1, ErbB4 and perlecan are present on pre-implantation embryos and blasto-cysts; interferon-{gamma} altered the expression of ErbB4 and Perlecan at the blastocyst stage. We propose that the effects produced by this cytokine could be related to the altered acquisition of adhesion competence and low implantation rates observed in certain reproductive immunological disorders.




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