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RESEARCH |
causes Ca2+ oscillations and parthenogenetic activation of human oocytes
1 Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, 2 Assisted Conception Unit and GKT Department of Womens Health, Guys and St Thomass NHS Trust, Guys Hospital, London SE1 9RT, 3 Wales Heart Research Institute, Wales College of Medicine, Heath Park, Cardiff University, Cardiff CF14 4XN and 4 Department of Obstetrics and Gynaecology, Wales College of Medicine, Heath Park, Cardiff University, Cardiff CF14 4XN, UK
Correspondence should be addressed to K Swann; Email: SwannK1{at}cf.ac.uk
At fertilization in mammals the sperm activates development of the oocyte by inducing a prolonged series of oscillations in the cytosolic free Ca2+ concentration. One theory of signal transduction at fertilization suggests that the sperm cause the Ca2+ oscillations by introducing a protein factor into the oocyte after gamete membrane fusion. We recently identified this sperm-specific protein as phospholipase C
(PLC
), and we showed that PLC
triggers Ca2+ oscillations in unfertilized mouse oocytes. Here we report that microinjection of the complementary RNA for human PLC
causes prolonged Ca2+ oscillations in aged human oocytes that had failed to fertilize during in vitro fertilization or intracytoplasmic sperm injection. The frequency of Ca2+ oscillations was related to the concentration of complementary RNA injected. At low concentrations, PLC
stimulated parthenogenetic activation of oocytes. These embryos underwent cleavage divisions and some formed blastocysts. These data show that PLC
is a novel parthenogenetic stimulus for human oocytes and that it is unique in its ability to mimic the repetitive nature of the Ca2+ stimulus provided by the sperm during human fertilization.
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