This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waterhouse, T. Articles by Shaw, J. Search for Related Content PubMed PubMed Citation Articles by Waterhouse, T. Articles by Shaw, J.

Offspring produced from heterotopic ovarian allografts in male and female recipient mice

¹ Department of Physiology, Monash University, Victoria 3800, Australia and ² Monash Institute of Reproduction and Development, 27-31 Wright Street, Clayton, Victoria 3168, Australia

Correspondence should be addressed to S-L Cox; Email: shae.cox{at}med.monash.edu.au

Studies on human ovarian xenografts and mouse allografts indicate that the male hormonal milieu and exogenous gonadotrophin administration stimulate antral follicle growth. However, it is not known whether oocytes produced under these conditions are developmentally competent. The objective of our study was to evaluate the developmental competence of oocytes produced in heterotopic mouse ovarian grafts placed in male and female recipient mice. Gonadotrophins were 7.5 IU pregnant mare serum gonadotrophin (PMSG) alone or 7.5 IU PMSG and 7.5 IU human chorionic gonadotrophin or were not given prior to oocyte collection. The developmental competence of oocytes was assessed by performing in vitro fertilisation and embryo transfer to recipients. When no gonadotrophins were given the cleavage rate was similar for oocytes collected from ovarian grafts in male and female recipients. Gonadotrophin treatment significantly (P < 0.05) increased two-cell formation by oocytes grown in female graft recipients but not in male recipients. Implantation rates, fetal development and the birth of live young were unaffected by the sex of the graft recipient or gonadotrophin treatment. Live offspring were produced from oocytes collected from ovarian grafts in male and female recipients treated with or without gonadotrophins. In conclusion, this work has shown that the hormonal environment of male mice can support the growth of oocytes in ovarian allografts and that these oocytes can produce live offspring.

This article has been cited by other articles:

				Y. Wang, Z. Xiao, L. Li, W. Fan, and S.-W. Li Novel needle immersed vitrification: a practical and convenient method with potential advantages in mouse and human ovarian tissue cryopreservation Hum. Reprod., July 9, 2008; (2008) den255v1. [Abstract] [Full Text] [PDF]

				W. Shen, D. Zhang, T. Qing, J. Cheng, Z. Bai, Y. Shi, M. Ding, and H. Deng Live Offspring Produced by Mouse Oocytes Derived from Premeiotic Fetal Germ Cells Biol Reprod, October 1, 2006; 75(4): 615 - 623. [Abstract] [Full Text] [PDF]

				H. Y. Yang, S.-L. Cox, G. Jenkin, J. Findlay, A. Trounson, and J. Shaw Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts. Reproduction, May 1, 2006; 131(5): 851 - 859. [Abstract] [Full Text] [PDF]