| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
RESEARCH |
Centre for Women’s Health Research, Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia
Correspondence should be addressed to J Girling; Email: jane.girling{at}med.monash.edu.au
The aim of this study was to investigate the role of intravascular neutrophils in initiating endothelial cell proliferation following oestrogen treatment in ovariectomised mouse endometrium. Uterine tissues were collected from ovariectomised C57/CBA female mice 24 h after oestrogen treatment with or without systemic neutrophil depletion. Neutropenia was achieved with either an in-house anti-neutrophil serum (ANS) or Gr-1 monoclonal antibody. All mice received an i.p. injection of bromodeoxyuridine (BrdU) 4 h prior to dissection to allow visualisation of proliferating cells using immunocytochemistry. Endometrial sections were immunostained for BrdU, vascular endothelial growth factor (VEGF), and neutrophils (using ANS). Oestrogen treatment of ovariectomised mice significantly increased the number of intravascular neutrophils, whereas induction of neutropenia with either ANS or Gr-1 in conjunction with oestrogen treatment prevented this increase. Oestrogen treatment of ovariectomised mice also significantly increased the number of intravascular VEGF-positive cells; however, whereas induction of neutropenia with ANS significantly reduced this increase, Gr-1 did not. In both studies, neutropenia significantly reduced, but did not eliminate, the amount of endometrial endothelial cell proliferation. These results suggest a role for neutrophils in endometrial angiogenesis following acute oestrogen treatment; however, the presence of VEGF-positive cells even after induction of neutropenia suggests that more than one type of leukocyte may be involved.
This article has been cited by other articles:
 |
|
 |
|
  J. E. Girling, F. L. Lederman, L. M. Walter, and P. A. W. Rogers Progesterone, But Not Estrogen, Stimulates Vessel Maturation in the Mouse Endometrium Endocrinology, November 1, 2007; 148(11): 5433 - 5441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Herington and B. M. Bany Effect of the Conceptus on Uterine Natural Killer Cell Numbers and Function in the Mouse Uterus During Decidualization Biol Reprod, April 1, 2007; 76(4): 579 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Na, S.-H. Yang, D.-W. Baek, D.-H. Lee, K.-H. Kim, Y.-M. Choi, S.-T. Oh, Y.-S. Hong, J.-Y. Kwak, and K.-S. Lee Effects of peritoneal fluid from endometriosis patients on the release of vascular endothelial growth factor by neutrophils and monocytes Hum. Reprod., July 1, 2006; 21(7): 1846 - 1855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W.S. Chan and C. E. Gargett Identification of Label-Retaining Cells in Mouse Endometrium Stem Cells, June 1, 2006; 24(6): 1529 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Albini, F. Tosetti, R. Benelli, and D. M. Noonan Tumor Inflammatory Angiogenesis and Its Chemoprevention Cancer Res., December 1, 2005; 65(23): 10637 - 10641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.A.W. Rogers, F. Martinez, J.E. Girling, F. Lederman, L. Cann, E. Farrell, F. Tresserra, and N. Patel Influence of different hormonal regimens on endometrial microvascular density and VEGF expression in women suffering from breakthrough bleeding Hum. Reprod., December 1, 2005; 20(12): 3341 - 3347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. McClintock and E. M. Wagner Role of IL-6 in systemic angiogenesis of the lung J Appl Physiol, September 1, 2005; 99(3): 861 - 866. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
