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Reproduction (2001) 121 541-546
DOI: 10.1530/rep.0.1210541
Copyright © 2001 Society for Reproduction and Fertility
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Articles

An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures

W Rens, F Yang, G Welch, S Revell, PC O'Brien, N Solanky, LA Johnson, and MA Ferguson Smith

X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. Clear hybridization signals were obtained for every spermatozoon using a modified sperm decondensation protocol and fluorescence in situ hybridization (FISH). The procedure was evaluated using the established Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry into X- and Y-bearing fractions. Close agreement was found between the assessment of sperm separation by flow cytometry and by FISH with the X-Y paint set. The FISH method is a simple, reliable and robust procedure for assessing the effectiveness of separation of X and Y spermatozoa.


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 Mol Hum ReprodHome page
L. Froenicke, P.-H. Hung, C. A. VandeVoort, and L. A. Lyons
Development of a non-human primate sperm aneuploidy assay tested in the rhesus macaque (Macaca mulatta)
Mol. Hum. Reprod., July 1, 2007; 13(7): 455 - 460.
[Abstract] [Full Text] [PDF]


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