This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Munir, I. Articles by Miyamoto, E. Search for Related Content PubMed Articles by Munir, I. Articles by Miyamoto, E.

Mitogen-activated protein kinase activation and regulation of cyclooxygenase 2 expression by platelet-activating factor and hCG in human endometrial adenocarcinoma cell line HEC-1B

The activation of mitogen-activated protein kinase (MAP kinase) and the regulation of cyclooxygenase 2 (COX-2) were investigated in the human endometrial adenocarcinoma cell line HEC-1B by treatment with platelet-activating factor (PAF) and hCG. Pre-treatment of the cells with both oestradiol and medroxyprogesterone acetate was required for MAP kinase activation and COX-2 expression to respond to PAF and hCG. PAF-induced MAP kinase activation was sensitive to MAP kinase kinase (MEK) inhibitor, PD098059, and phosphatidylinositol-3-OH kinase (PI3K) inhibitor, wortmannin. In contrast, hCG-induced MAP kinase activation was sensitive to PD098059 and protein kinase A inhibitor, H-89, but not to wortmannin. PAF-induced COX-2 expression was insensitive to PD098059 but sensitive to wortmannin, whereas hCG-induced COX-2 expression was sensitive to PD098059 and H-89 but insensitive to wortmannin. 8-(4-chlorophenylthio)-cAMP, a potent cAMP analogue, induced activation of MAP kinase and expression of COX-2. These results indicate that MAP kinase is activated with PAF and hCG in HEC-1B cells. In addition, COX-2 expression is stimulated through the MAP kinase activation pathway with hCG and the wortmannin sensitive pathway with PAF in HEC-1B cells. These results also imply that protein kinase A remains upstream of hCG-induced activation of MAP kinase in HEC-1B cells.

This article has been cited by other articles:

				W. Murk, C. S. Atabekoglu, H. Cakmak, A. Heper, A. Ensari, U. A. Kayisli, and A. Arici Extracellularly Signal-Regulated Kinase Activity in the Human Endometrium: Possible Roles in the Pathogenesis of Endometriosis J. Clin. Endocrinol. Metab., September 1, 2008; 93(9): 3532 - 3540. [Abstract] [Full Text] [PDF]

				T. Harada, H. Kanasaki, S. Mutiara, A. Oride, and K. Miyazaki Cyclic Adenosine 3',5'Monophosphate/Protein Kinase A and Mitogen-Activated Protein Kinase 3/1 Pathways Are Involved in Adenylate Cyclase-Activating Polypeptide 1-Induced Common Alpha-Glycoprotein Subunit Gene (Cga) Expression in Mouse Pituitary Gonadotroph LbetaT2 Cells Biol Reprod, October 1, 2007; 77(4): 707 - 716. [Abstract] [Full Text] [PDF]

				E. Dos Santos, M.-N. Dieudonne, M.-C. Leneveu, R. Pecquery, V. Serazin, and Y. Giudicelli In vitro effects of chorionic gonadotropin hormone on human adipose development J. Endocrinol., August 1, 2007; 194(2): 313 - 325. [Abstract] [Full Text] [PDF]

				Y. Yamada, H. Yamamoto, T. Yonehara, H. Kanasaki, H. Nakanishi, E. Miyamoto, and K. Miyazaki Differential Activation of the Luteinizing Hormone {beta}-Subunit Promoter by Activin and Gonadotropin-Releasing Hormone: A Role for the Mitogen-Activated Protein Kinase Signaling Pathway in L{beta}T2 Gonadotrophs Biol Reprod, January 1, 2004; 70(1): 236 - 243. [Abstract] [Full Text] [PDF]

				S. Srisuparp, Z. Strakova, A. Brudney, S. Mukherjee, S. Reierstad, M. Hunzicker-Dunn, and A. T. Fazleabas Signal Transduction Pathways Activated by Chorionic Gonadotropin in the Primate Endometrial Epithelial Cells Biol Reprod, February 1, 2003; 68(2): 457 - 464. [Abstract] [Full Text] [PDF]

				M. Zygmunt, F. Herr, S. Keller-Schoenwetter, K. Kunzi-Rapp, K. Munstedt, C. V. Rao, U. Lang, and K. T. Preissner Characterization of Human Chorionic Gonadotropin as a Novel Angiogenic Factor J. Clin. Endocrinol. Metab., November 1, 2002; 87(11): 5290 - 5296. [Abstract] [Full Text] [PDF]

				T. Yonehara, H. Kanasaki, H. Yamamoto, K. Fukunaga, K. Miyazaki, and E. Miyamoto Involvement of Mitogen-Activated Protein Kinase in Cyclic Adenosine 3',5'-Monophosphate-Induced Hormone Gene Expression in Rat Pituitary GH3 Cells Endocrinology, July 1, 2001; 142(7): 2811 - 2819. [Abstract] [Full Text] [PDF]

				I. Munir, K. Fukunaga, H. Kanasaki, K. Miyazaki, T. Ohba, H. Okamura, and E. Miyamoto Expression of Cyclooxygenase 2 by Prostaglandin E2 in Human Endometrial Adenocarcinoma Cell Line HEC-1B Biol Reprod, September 1, 2000; 63(3): 933 - 941. [Abstract] [Full Text]

				V. R. Sunil, A. J. Connor, Y. Guo, J. D. Laskin, and D. L. Laskin Activation of type II alveolar epithelial cells during acute endotoxemia Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L872 - L880. [Abstract] [Full Text] [PDF]