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THE EFFECTS OF PURIFIED SHEEP LUTEINIZING HORMONE ON THE GUINEA-PIG OVARY

Luteinizing hormone (Ovine, NIH-LH-S7 from the National Institutes of Health, Bethesda, U.S.A.) has been administered subcutaneously in normal saline (0·5 mg/ml) to female guinea-pigs for a study of its effects on ovulation and the cycle.

The animals were examined daily for vaginal changes; the first day of vaginal closure after oestrus (corresponding in general to the morning of the vaginal plug in mated females), was recorded as Day 0 and the following days as (luteal) Days 1, 2, etc. In most experiments a single dose of LH was given; the ovaries were examined at autopsy, and one or both sectioned serially after fixation in Bouin's fluid. Preliminary tests in cyclic females showed that 200 µg LH would stimulate ovulation within 24 hr and also follicular luteinization.

Fifteen unmated non-parous females with a regular cycle of about 16