This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Goetz, F. W. Articles by Bradley, J. A. Search for Related Content PubMed PubMed Citation Articles by Goetz, F. W. Articles by Bradley, J. A.

Stimulation of in vitro ovulation and contraction of brook trout (Salvelinus fontinalis) follicles by adrenaline through -adrenoreceptors

The effects of adrenaline and adrenoreceptor antagonists on ovulation and follicle wall contraction were investigated in brook trout (Salvelinus fontinalis) follicles using in vitro incubation systems. Adrenaline significantly stimulated a dose-dependent increase in ovulation and follicle contraction at concentrations between 1.0 and 100 µmol l^–1 The ovulatory and contractile effects of 10 µmol adrenaline l^–1 could be blocked by the ₁-adrenoreceptor antagonists WB-4101 and benoxathian, and by the ₂-antagonist yohimbine. WB-4101 was the most potent blocker, significantly inhibiting ovulation and contraction at 1.0 µmol l^–1. In contrast, the β-antagonist propranolol (100–0.001 µmol l^–1) was totally ineffective in blocking adrenaline-induced ovulation and follicle contraction. The results indicate that there is a strong correlation between the effects of adrenaline on ovulation and contraction. In addition, the antagonist studies indicate that adrenaline stimulates ovulation and follicle contraction of brook trout follicles through -adrenoreceptors.